Evaluation of the Genotoxicity of Endodontic Materials for Deciduous Teeth Using the Comet Assay
نویسندگان
چکیده
Objective: To evaluate genotoxicity of zinc oxide, P. A. calcium hydroxide, mineral trioxide aggregate and an iodoform paste using comet assay on human lymphocytes. Material Methods: Two positive controls were used: methyl-methanesulfonate for the P.A. hydroxide aggregate; doxorubicin oxide. There also two negative controls: distilled water DMSO Comets identified fluorescence microscopy 100 them counted each three slides analyzed per drug test. A damage index was established, taking into consideration score pattern that had previously been determined from size intensity tail. Analysis variance, followed by Tukey’s test, used to compare means DNA indices. Results: The observed for (7.08 8.58) (6.50 8.33), which similar control index. On other hand, oxide (104.7 218.50) (115.7 210.7) Conclusion: Iodoform showed at all concentrations used.
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ژورنال
عنوان ژورنال: Brazilian Research in Pediatric Dentistry and Integrated Clinic
سال: 2021
ISSN: ['1983-4632', '1519-0501']
DOI: https://doi.org/10.1590/pboci.2021.062